p50 pcdna3 expression vectors Search Results


plasmids pcdna3 1 p50  (Addgene inc)
85
Addgene inc plasmids pcdna3 1 p50
Plasmids Pcdna3 1 P50, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/pcDNA-p50+(Plasmid+%23107978)/pmc02804168-270-11-15
Average 85 stars, based on 1 article reviews
plasmids pcdna3 1 p50 - by Bioz Stars, 2026-09
85/100 stars
  Buy from Supplier
p50  (Addgene inc)
93
Addgene inc p50
FIGURE2.ModulationofSDC4promoteractivityandNF-BsignalingbycytokinesinNPcells.TreatmentwithbothTNF-(A)andIL-1(B)inducesactivity of SDC4 promoter in NP cells. C, a combined treatment of TNF- and IL-1 shows no additive or synergistic effect on induction of SDC4 promoter activity over individual cytokine treatments. D, NP cells were co-transfected with RelB or cRel and SDC4 promoter activity measured. Only cRel has a small inductive effect on SDC4 promoter activity. Values shown are mean S.E., of three independent experiments; *, p 0.05. E and F, Western blot analysis of NF-B signaling proteins, p65 and <t>p50</t> following treatment of NP cells with TNF- (E) and IL-1 (F). Cytokine treatment induced phosphorylation of p65 within first 15 min and levels remained elevated after 24 h. No appreciable change in expression of p65 and p50 was seen. G, immunofluorescent analysis of NP cells treated with TNF- and IL-1. Cells showed increased nuclear localization of both phospho-p65 and p65 whereas p50 expression and localization did not change.
P50, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/p50+cFlag+pcDNA3+(Plasmid+%2320018)/10__1074_slash_jbc__m111__264549-51-7-31
Average 93 stars, based on 1 article reviews
p50 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
pcdna3.1-nfkb1 plasmid  (Shanghai GenePharma)
90
Shanghai GenePharma pcdna3.1-nfkb1 plasmid
miR-450b-5p targets <t>NFKB1.</t> (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.
Pcdna3.1 Nfkb1 Plasmid, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/pcdna+p65+plasmid/pmc09366159-32-32-37
Average 90 stars, based on 1 article reviews
pcdna3.1-nfkb1 plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
full length cflag pcdna3 nfκb isoforms codifying plasmids  (Addgene inc)
92
Addgene inc full length cflag pcdna3 nfκb isoforms codifying plasmids
miR-450b-5p targets <t>NFKB1.</t> (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.
Full Length Cflag Pcdna3 Nfκb Isoforms Codifying Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/p52+cFlag+pcDNA3+(Plasmid+%2320019)/pmc03356824-312-2-15
Average 92 stars, based on 1 article reviews
full length cflag pcdna3 nfκb isoforms codifying plasmids - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
p50 pcdna3 expression vectors  (Addgene inc)
93
Addgene inc p50 pcdna3 expression vectors
miR-450b-5p targets <t>NFKB1.</t> (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.
P50 Pcdna3 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/RelA+cFlag+pcDNA3+(Plasmid+%2320012)/pmc05371897-234-18-28
Average 93 stars, based on 1 article reviews
p50 pcdna3 expression vectors - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
c src kinase  (Addgene inc)
93
Addgene inc c src kinase
miR-450b-5p targets <t>NFKB1.</t> (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.
C Src Kinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p50+pcdna3+expression+vectors/pcDNA3+c-SRC+(WT)+(Plasmid+%2342202)/pm26403203-116-27-32
Average 93 stars, based on 1 article reviews
c src kinase - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
nano-lantern(ca2+)_150/pcdna3 (plasmid #51984)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier
genl(ca2+)_250/pcdna3 (plasmid #85206)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier
p5065 pcdna3.1/v5-his-topo hbrd4 (888-1362) (plasmid #14444)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier
p5064 pcdna3.1/v5-his-topo hbrd4 (1-461) (plasmid #14443)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier
yellow cameleon-nano50/pcdna3 (plasmid #51964)  (Addgene inc)
N/A
   Buy from Supplier
airap 5: airap(1-50).gst,v1.pcdna3 (plasmid #21795)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
   Buy from Supplier

Image Search Results


FIGURE2.ModulationofSDC4promoteractivityandNF-BsignalingbycytokinesinNPcells.TreatmentwithbothTNF-(A)andIL-1(B)inducesactivity of SDC4 promoter in NP cells. C, a combined treatment of TNF- and IL-1 shows no additive or synergistic effect on induction of SDC4 promoter activity over individual cytokine treatments. D, NP cells were co-transfected with RelB or cRel and SDC4 promoter activity measured. Only cRel has a small inductive effect on SDC4 promoter activity. Values shown are mean S.E., of three independent experiments; *, p 0.05. E and F, Western blot analysis of NF-B signaling proteins, p65 and p50 following treatment of NP cells with TNF- (E) and IL-1 (F). Cytokine treatment induced phosphorylation of p65 within first 15 min and levels remained elevated after 24 h. No appreciable change in expression of p65 and p50 was seen. G, immunofluorescent analysis of NP cells treated with TNF- and IL-1. Cells showed increased nuclear localization of both phospho-p65 and p65 whereas p50 expression and localization did not change.

Journal: Journal of Biological Chemistry

Article Title: TNF-α and IL-1β Promote a Disintegrin-like and Metalloprotease with Thrombospondin Type I Motif-5-mediated Aggrecan Degradation through Syndecan-4 in Intervertebral Disc

doi: 10.1074/jbc.m111.264549

Figure Lengend Snippet: FIGURE2.ModulationofSDC4promoteractivityandNF-BsignalingbycytokinesinNPcells.TreatmentwithbothTNF-(A)andIL-1(B)inducesactivity of SDC4 promoter in NP cells. C, a combined treatment of TNF- and IL-1 shows no additive or synergistic effect on induction of SDC4 promoter activity over individual cytokine treatments. D, NP cells were co-transfected with RelB or cRel and SDC4 promoter activity measured. Only cRel has a small inductive effect on SDC4 promoter activity. Values shown are mean S.E., of three independent experiments; *, p 0.05. E and F, Western blot analysis of NF-B signaling proteins, p65 and p50 following treatment of NP cells with TNF- (E) and IL-1 (F). Cytokine treatment induced phosphorylation of p65 within first 15 min and levels remained elevated after 24 h. No appreciable change in expression of p65 and p50 was seen. G, immunofluorescent analysis of NP cells treated with TNF- and IL-1. Cells showed increased nuclear localization of both phospho-p65 and p65 whereas p50 expression and localization did not change.

Article Snippet: Expression plasmids pCMX-I BM (#12330), RelA/p65 (#20012), p50 (#20018), developed by Dr. Inder Verma, RelB (#20017) developed by Dr.Thomas Gilmore and c-Rel (27253) developed by Dr. Stephen Smale were obtained from Addgene.

Techniques: Activity Assay, Transfection, Western Blot, Phospho-proteomics, Expressing

miR-450b-5p targets NFKB1. (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.

Journal: Molecular Medicine Reports

Article Title: Knockdown of hsa_circ_0005699 attenuates inflammation and apoptosis induced by ox-LDL in human umbilical vein endothelial cells through regulation of the miR-450b-5p/NFKB1 axis

doi: 10.3892/mmr.2022.12806

Figure Lengend Snippet: miR-450b-5p targets NFKB1. (A) StarBase online database analysis revealed that mir-450b-5p targets NFKB1. (B) Dual-luciferase reporter assay verified the targeting of miR-450b-5P to NFKB1. **P<0.01 vs. miR-NC. (C) mRNA and (D) protein expression levels of NFKB1 after circ_0005699 knockdown, as determined by reverse transcription-quantitative PCR and western blotting. **P<0.01 vs. sh-NC. miR, microRNA; Mut, mutant; NC, negative control; sh, short hairpin RNA; WT, wild-type.

Article Snippet: pLKO.1-puro was used as the backbone for the shRNA constructs. circ_0005699 short hairpin RNA (sh-circ_0005699#1/sh-circ_0005699#2), negative control shRNA (sh-NC), miR-450b-5p mimics and negative control (mimics-NC), miR-450b-5p inhibitor and negative control (inhibitor-NC) and pcDNA3.1-NFKB1 plasmid were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Luciferase, Reporter Assay, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Mutagenesis, Negative Control, shRNA

circ_0005699 attenuates HUVEC apoptosis and inflammation through the miR-450b-5p/NFKB1 axis. (A) Protein expression levels of NFKB1 in different groups of HUVECs, as detected by western blotting. (B) Cell Counting Kit-8 analysis of HUVEC proliferation following different treatments. (C) Apoptotic rate of HUVECs in different groups, as determined by Annexin V/PI double staining and flow cytometry. (D) Protein expression levels of apoptosis-related genes Bcl-2 and Bax. **P<0.01 vs. sh-NC, ##P<0.01 vs. sh-circ_0005699#1. Bcl-2, B-cell lymphoma 2; HUVEC, human umbilical vein endothelial cell; miR, microRNA; NC, negative control; sh, short hairpin RNA.

Journal: Molecular Medicine Reports

Article Title: Knockdown of hsa_circ_0005699 attenuates inflammation and apoptosis induced by ox-LDL in human umbilical vein endothelial cells through regulation of the miR-450b-5p/NFKB1 axis

doi: 10.3892/mmr.2022.12806

Figure Lengend Snippet: circ_0005699 attenuates HUVEC apoptosis and inflammation through the miR-450b-5p/NFKB1 axis. (A) Protein expression levels of NFKB1 in different groups of HUVECs, as detected by western blotting. (B) Cell Counting Kit-8 analysis of HUVEC proliferation following different treatments. (C) Apoptotic rate of HUVECs in different groups, as determined by Annexin V/PI double staining and flow cytometry. (D) Protein expression levels of apoptosis-related genes Bcl-2 and Bax. **P<0.01 vs. sh-NC, ##P<0.01 vs. sh-circ_0005699#1. Bcl-2, B-cell lymphoma 2; HUVEC, human umbilical vein endothelial cell; miR, microRNA; NC, negative control; sh, short hairpin RNA.

Article Snippet: pLKO.1-puro was used as the backbone for the shRNA constructs. circ_0005699 short hairpin RNA (sh-circ_0005699#1/sh-circ_0005699#2), negative control shRNA (sh-NC), miR-450b-5p mimics and negative control (mimics-NC), miR-450b-5p inhibitor and negative control (inhibitor-NC) and pcDNA3.1-NFKB1 plasmid were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Expressing, Western Blot, Cell Counting, Double Staining, Flow Cytometry, Negative Control, shRNA